Blood Smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. An IUD does not interfere with the ability to get a good sample. 30×) than in an equal area of a thin smear. Use either Push wedge method or Automated method to prepare blood smear. Ann Biol Clin (Paris). Different Dyes stain various cell componets with different color. Increase in the amout of thrombocytes, seen in blood loss and after surgery. Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. Exercise 9: Preparation of Peripheral Blood Smears Blood viscosity (thickness of blood) will vary from patient to patient. If RBCs are bright red it indicates stain or buffer is too acid, drying is incomplete before staining, Staining time is insufficient or inadequate. Has both thick and thin areas with gradual transition. Does not cover the entire area of the slide. Purpose and Criteria for Blood Smear Scan, Blood Smear Examination, and Blood Smear Review.pdf Available via license: CC BY-NC 3.0 Content may be subject to copyright. 3. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Characteristics of A Good Smear 1. Describe the blood specimen and procedure for preparing a blood smear. Microcytic when the size is smaller than normal RBC and these are less than 6 µm. 1. 3. Characteristics of good smear : 1.Surface should be even and uniform ,margins should not extend to the side of the slide. 2. Proper Preparation of a Peripheral Blood Smear Objectives At the completion of this laboratory, you will be able to: 1. See our User Agreement and Privacy Policy. It is often done as part of or along with a complete blood count (CBC). Now customize the name of a clipboard to store your clips. 3. Peripheral Blood Smear DR. ANWAR H. SIDDIQUI 2. No area contains large amounts of broken cells or precipitated stain. The peripheral blood film remains a very useful tool in hematological assessment. Explain the factors, which affect the quality of stained blood smear, including sources of error in the technique. PROCEDURE FOR PREPARING AN IDEAL SMEAR ⇒ Take a Clean, Grease free microscopic glass slide and mark the smear area on the underside of the slide with a glass marking pencil. A blood smear evaluates red blood cells (RBC morphology) white blood cells (WBC) and platelets to help diagnose Blood smears are used to determine leukocyte differentials, to evaluate erythrocyte, platelet and leukocyte morphology, and, if necessary, to estimate platelet and leukocyte counts. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. In peripheral blood film (PBF), neutrophils showed characteristic C-shaped, fetus-like nuclei, elongated nucleoplasm, and ring-shaped nuclei. Thick smears The blood elements (including parasites, if any) are more concentrated (app. If you are using venous blood, blood smears should be prepared as soon as possible after collection (delay can result in changes in parasite morphology and staining characteristics). Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Bone Marrow (not performed by IDC). If you continue browsing the site, you agree to the use of cookies on this website. However, microscopic evaluation of peripheral blood smears affords clinicians additional data that may be used to guide diagnosis and treatment. Improper timing of staining or buffering, Deteriorated reagent. You can have a pap smear with an IUD in place. Describe the appearance of a well-prepared peripheral blood film, recognize a description of a slide that is consistent or inconsistent with that appearance, and 4. Normochromic when the color is normal. Peripheral Blood Smear 1. Flame the slide and place it on the disinfected table. Abnormal results may vary depending on the type of blood cell affected. 2. Describe the optimal area for evaluating and enumerating blood cells on a well stained smear. RBCs are not quite touching and have a central pallor. Any condition which causes an increase in the Sufficient reading area ADVERTISEMENTS: ii. Anemia, morphology, red blood cell, poikilocytosis, peripheral smear SUMMARY The foundation of laboratory hematologic diagnosis is the complete blood count and review of the peripheral smear. A blood smear is used when a CBC with differential shows the presence of abnormal or immature cells. Clinical Case Study Fatigued: A Case on Blood Download Case Study with Worksheet Harold is a 67-year-old male who has had regular physical exams, is a non-smoker, and who has been in good health for most of his life. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… The shape of blood film 9. Serum folate and vitamin B 12 were normal, but plasma homocysteine was elevated (>100.0 μmol/L), methionine decreased (10 μmol/L), and methylmalonic acid normal, indicating a specific homocysteine to methionine conversion defect. Large granular lymphocytes noted, a representation of natural killer cells or cytotoxic T lymphocytes. A good wedge smear should cover half to three fourths of the slide, should show gradual transition from a thick to a thin end with a feathered edge. 4. Left smear is unstained, right smear is stained with Wright-Giemsa stain. 2.Smear should be tongue shaped . Burkitt cells on a peripheral blood smear: how to deal with? Looks like you’ve clipped this slide to already. Attention to detail in slide preparation, staining and standard criteria for cell estimates, morphology and differential reporting enhance the value of Normal, mature RBCs are biconcave, disc-shaped, anuclear cells measuring approximately 7-8 microns in diameter on a peripheral blood smear with an internal volume of 80-100 femtoliters (fL). Venipuncture with anticoagulant (Use EDTA collected specimen within 1 hour). List the causes of a poor blood smear preparation. A pap smear does not affect your fertility. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Does not State the appropriate sample used for preparing a peripheral blood smear. Parts of the Compound Microscope Tube It is cylindrical tube through which the light from an illuminated object traverses enroute its formation of an image. Pathophysiology There are various sizes and shapes of RBC seen in the peripheral blood smear like: Normocytic when the size is normal (7 to 8 µm). Understand the purpose of performing a peripheral blood smear examination. A peripheral blood smear is a glass microscope slide coated on one side with a thin layer of venous blood.The slide is stained with a dye, usually Wright’s stain, and examined under a microscope[1]. Two push-type peripheral blood smears suitable for characterization of cellular blood elements. List the characteristics of a good blood smear. A lymphocyte is a type of white blood cell in the vertebrate immune system. Compound Microscope 1 3. Prolonged storage of anticoagulated blood specimen, Delay in spreading the blood drop, Dirty and poor quality glass slide, Inappropriate size of blood drop, Improper angle of the pusher slide, Improper pressure/speed, High Humidity. Peripheral Decrease in the amount of thrombocytes seen in acute leukemia , radiation therapy. Bacteria, Babesia or Malaria, Stain Precipitates. Describe the appearance of a It consists of an outer body tube and an inner draw tube which can slide inside the outer, eye piece and lower part carrying a revolving … Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood … All liver function tests were abnormal indicating that the target cells are due to liver disease secondary to CD. 11. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, … In this video I quickly go over how to make a peripheral blood smear. blood smear. DR. ANWAR H. SIDDIQUI. You can change your ad preferences anytime. Cell Size (immature cells are larger), Nuclear to cytoplasmic ratio (relative and absoluate size of the nucleus decreases as a result of nuclear maturation) , RBC's (nucleus and other organelles disappear), Nucleus (Young nucleus is reddish and bcome bluer as cell ages, chromatin is fine and lacy and becomes coarse, clumped and condensed as cells mature), Cytoplasm (Granulocyte matures from non granulated to specific granules which differs in dye affinity, granules increase in number). Peripheral blood smear of a 38-year-old female with long-standing Crohn’s disease (CD) and development of microcytic anemia. A blood smear is considered abnormal when there’s an abnormality in the size, shape, color, or number of cells in your blood. Clipping is a handy way to collect important slides you want to go back to later. If red blood cells appear blueish or green indicates stain or buffer is too alkaline, smear is too thick, Prolonged Staining. 2016 Dec 1;74(6):697-703 Authors: Wyrwas M, Michel J, Guibaud I, Lesesve JF, Bénet B Abstract The diagnosis of double hit lymphoma remains a challenge for the biologist for a good management of the patient. A blood smear is often used as a follow-up test to abnormal results on a total blood count (CBC) to evaluate the various types of blood cells. The peripheral blood smear was consistent with a leukoerythroblastic pic-ture with normocytic A. LIst the components of peripheral blood smear examination. Osmotic fragility & rbc membrane defects 050916, No public clipboards found for this slide. Describe the techniques for making peripheral blood films. A peripheral smear test can be used to diagnose, monitor numerous conditions and blood diseases that affect population of blood cells. Understand the general rule of cell identification. Making a great blood smear (or blood film as some call it) is an important skill for any veterinary professional, even if your hospital does not require differentials to be done on a regular basis.It’s a great way to impress your colleagues! Desirable qualities of a blood smear include: i. cells, prompting peripheral blood smear evaluation. It should have a smooth appearance and have no ridges, holes, lines, streaks or clumps. Two main conditions will affect the viscosity of the blood. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters like complete blood count or CBC can be obtained within a few minutes. Good smear is tongue shaped with a smooth tail. Fingerstick- Best sample (no anticoagulant effect), 2. [1] Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive immunity). Mild monocytosis (900/μL) along with the persistent lymphopenia was also noted. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. There are many reasons why your doctor may order a blood smear. Describe different erythrocyte inclusions, their etiology and their clinical significance. In patients with anemia, the A polychrome stain, contains methylene blue and eosine dye dissolved in methyl alcohol. The blood smear demonstrated hypersegmented neutrophils (panels A,C), macrocytes (panel B), and red blood cell fragments (panels A,C). Erythrocyte Morphology, Platelet evaluation, Quantitative White Blood Cells. The smear shows numerous target cells and a spur cell (top right). Examining the monolayer of a well-prepared and properly stained blood smear allows veterinary technicians to assess erythrocyte (RBC), leukocyte (WBC) and platelet morphology. 1. It might be used to help identify and/or keep an eye on numerous conditions See our Privacy Policy and User Agreement for details. If you continue browsing the site, you agree to the use of cookies on this website. A blood smear is a blood test that gives information about the number and shape of blood cells. State the components and function of a Wright's Stain. To determine white blood cells population and distribuation is within normal limits, and also assess the RBC and platelet morphology. The manual peripheral blood smear Whilst the manual differential count is generally considered to be superior to an analyser generated count for pathologi-cal samples, this only holds true under very strict conditions. 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